ROTAPREP SAMPLE PREPARATION
PolyLyser
Rapid benchtop homogenization with HyperLysing technology
Process multiple tube formats with full-length bead motion, programmable touchscreen control, and cryogenic or room-temperature preparation.
- Supports standard 2 mL, 15 mL, and 50 mL tubes with the appropriate platform
- Adjustable speed from 600–3,000 RPM and run times up to 60 seconds
- Full-length bead travel with HyperLysing technology
- Integrated dry-ice holder for cryogenic processing
How the PolyLyser Works
The PolyLyser uses HyperLysing technology to move the sample tube in a controlled motion that drives the lysing matrix from one end of the tube to the other. This end-to-end bead motion mechanically disrupts samples for downstream DNA, RNA, and protein extraction.
Efficient grinding can reduce the processing time needed to break down a sample. Select the lysing matrix and run conditions for your material and the molecules you want to recover.
Learn about RotaPrep’s HyperLysing technology →The touchscreen lets you store and recall processing profiles. Tube platforms support cryogenic and room-temperature preparation for frozen and temperature-sensitive samples.
Applications and Sample Types
Sample Types
- Plant tissue, seeds, and agricultural materials
- Animal tissue and snap-frozen biopsies
- Bacteria, yeasts, fungi, and spores
- Gut, fecal, soil, and environmental samples
- Clinical, forensic, archaeological, and paleontological samples
Application Areas
- DNA, RNA & protein extraction from plants, animals, fungi, and bacteria
- Cryogenic lysis of snap-frozen biopsies and tissues
- Microbiome profiling from gut, fecal, and soil samples
- Spore lysis and pathogen detection
- Metabolomics & proteomics sample prep
- Chromatin shearing for ChIP and NGS
- Clinical, forensics, and archaeological samples
- Environmental and paleontological sample disruption
Who It’s For
- Molecular biologists working to protect RNA from thermal degradation
- Microbiome scientists working with fecal and soil DNA
- Core lab managers handling high-throughput pipelines
- Educators updating student sample-preparation laboratories
- Pharma and biotech researchers developing reproducible lysis for omics workflows
Downstream Workflows
Use an appropriate extraction and purification method for your sample and target molecule:
- TRIzol® or magnetic bead kits
- Column-based extractions
- qPCR, RT-qPCR, NGS, or RNA-seq
- Western blots or mass spectrometry
- Microbiome, metagenomic, and proteomic analyses
Rapid Sample Preparation at the Bench
Multiple Samples
Process up to twelve standard 2 mL screw-cap tubes in a single run, with platforms for larger tubes as well.
Cryogenic Processing
Use cryogenic or room-temperature processing to suit your samples, including frozen and temperature-sensitive materials.
Touchscreen Control
Set processing conditions through the touchscreen and store profiles for recall in repeat workflows.
Simple to Use
Build a repeatable workflow around your sample type and extraction method.
Prepare Your Samples
Select compatible tubes, the appropriate platform, and a lysing matrix suited to your sample.
Select Your Settings
Use the touchscreen to set processing conditions or recall a stored profile. Load and operate the instrument according to its instructions.
Continue Extraction
After homogenization, proceed with the extraction, purification, or analysis method appropriate for your sample.
Processing time depends on the sample, tube format, grinding media, and protocol. Optimize conditions for your downstream workflow.
Specifications at a Glance
| Product | PolyLyser benchtop homogenizer |
|---|---|
| Product code | PLYS-012 |
| Lysing technology | Patent-pending HyperLysing full-length bead motion |
| 2 mL capacity | Up to 12 standard screw-cap tubes per run |
| Larger tube platforms | 2 × 15 mL or 2 × 50 mL tubes |
| Speed range | 600–3,000 RPM |
| Run time | Up to 60 seconds |
| Temperature options | Room-temperature processing or cryogenic processing with the integrated dry-ice holder |
| Controls | Touchscreen with programmable profile memory |
| Manufacturing | Designed and assembled in Tustin, California, USA |
PolyLyser Resources
Explore sample applications, tube platforms, and guidance for planning your workflow.
Applications and Sample Types
Biological Samples
- Plant tissues and seeds
- Animal tissues and frozen biopsies
- Bacteria, yeasts, fungi, and spores
Environmental Samples
- Soil and fecal samples
- Gut and environmental microbiome samples
- Agricultural and other challenging materials
Downstream Workflows
Prepare lysates for DNA, RNA, protein, and metabolite extraction. Choose a compatible purification method, such as a column-based, magnetic-bead, or reagent-based protocol.
Purified material may be used in workflows such as qPCR, RT-qPCR, sequencing, western blotting, and mass spectrometry, subject to the quality requirements of the selected method.
Temperature-Sensitive Samples
Cryogenic processing provides an option for frozen samples and workflows where controlling heat is important. Select processing and extraction conditions appropriate for RNA, proteins, and other sensitive targets.
Choose a Platform for Your Tubes
| Tube size | Capacity per run | Format |
|---|---|---|
| 2 mL | 12 tubes | Standard screw-cap lysing tubes |
| 15 mL | 2 tubes | Compatible screw-cap tubes |
| 50 mL | 2 tubes | Compatible screw-cap tubes |
Use the matching platform for each tube format. Confirm platform selection and tube compatibility when ordering.
Cryogenic and Room-Temperature Use
The tube platforms support both cryogenic and room-temperature processing. Select the mode and preparation procedure that fit your sample and extraction protocol.
Selecting a Lysing Matrix
Choose the bead material and size for your sample. Different tissues and microorganisms can require different grinding conditions, even when they use the same tube format.
Operating Instructions and Protocol Support
Contact RotaPrep for operating instructions, tube-platform selection, and help planning a PolyLyser workflow. Have your sample type, tube size, and intended extraction method available so we can help identify the appropriate setup.
Understand the Lysing Motion
Learn how full-length bead travel supports mechanical sample disruption.
Explore HyperLysingPolyLyser Technical Summary
The PolyLyser is a programmable benchtop homogenizer for standard screw-cap tube formats. Its patent-pending HyperLysing motion drives the lysing matrix through the full length of the sample tube.
| Supported formats | Up to 12 × 2 mL tubes; 2 × 15 mL tubes; or 2 × 50 mL tubes, using the appropriate platform |
|---|---|
| Speed range | 600–3,000 RPM |
| Run time | Up to 60 seconds |
| Temperature control | Room-temperature processing or cryogenic processing with the integrated dry-ice holder |
| Controls | Touchscreen with programmable profile memory |
| Applications | Mechanical sample disruption before DNA, RNA, protein, metabolite, and related analytical workflows |
Performance Note
Processing time and recovery depend on the sample, tube format, grinding media, temperature, and downstream method. Optimize and validate conditions for each workflow.
Ordering and Protocol Support
Contact RotaPrep to confirm the required platform, tube compatibility, and recommended starting conditions for your samples.