Protected innovation

HyperLysing technology patents

Six issued U.S. patents document the mechanical architecture and motion systems behind RotaPrep’s fast sample-grinding and homogenization technology.

2017 Issued patent
US 9,759,638 B1

Apparatus and Method for Grinding of Samples for Analysis

InventorHoracio Kido IssuedSeptember 12, 2017
2019 Issued patent
US 10,502,667 B2

Apparatuses for Generating a Reciprocating Motion for the Purpose of Grinding of Samples

InventorHoracio Kido IssuedDecember 10, 2019
2020 Issued patent
US 10,539,486 B2

Methods for Grinding of Samples Using a Combination of Rotational and Linear Motion

InventorHoracio Kido IssuedJanuary 21, 2020
2022 Issued patent
US 11,474,006 B2

Methods for Grinding of Samples Using a Combination of Rotational and Linear Motion

InventorHoracio Kido IssuedOctober 18, 2022
2024 Issued patent
US 11,921,019 B2

Grinding Samples Using Rotational and Linear Motion

InventorHoracio Kido IssuedMarch 5, 2024
2025 Issued patent
US 12,298,209 B2

Grinding Samples Using Rotational and Linear Motion

InventorHoracio Kido IssuedMay 13, 2025

Published applications

Publication & reference library

ML References for MonoLyserResearch and technical literature citing the platform 17 references
  1. Kido, Horacio. “Hand-Held Homogenization Tool: New System Can Be Used in Field or Laboratory.” Genetic Engineering & Biotechnology News 33.8 (2013): 24-25.

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  2. Kido, Horacio. “Portable Systems for Sample Lysis and Homogenization.” Sample Preparation Techniques for Soil, Plant, and Animal Samples. New York, NY: Springer New York, 2016. 117-123.

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  3. Ahmed, Bilal, et al. “Development of a Portable Paper-based Biosensor for the Identification of Genetically Modified Corn (Zea mays) and Soybean (Glycine max).” Biosensors and Bioelectronics (2025): 117690.

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  4. Huang, Xiang-Feng, et al. “Culture strategies for lipid production using acetic acid as sole carbon source by Rhodosporidium toruloides.” Bioresource technology 206 (2016): 141-149.

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  5. Liu, Jia, et al. “Bioconversion of mixed volatile fatty acids into microbial lipids by Cryptococcus curvatus ATCC 20509.” Bioresource Technology 241 (2017): 645-651.

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  6. Huang, Xiangfeng, et al. “Enhancement of lipid accumulation by oleaginous yeast through phosphorus limitation under high content of ammonia.” Bioresource Technology 262 (2018): 9-14.

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  7. Liu, Jia, et al. “Microbial conversion of mixed volatile fatty acids into microbial lipids by sequencing batch culture strategy.” Bioresource Technology 222 (2016): 75-81.

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  8. Huang, Xiang-Feng, et al. “Using non-ionic surfactant as an accelerator to increase extracellular lipid production by oleaginous yeast Cryptococcus curvatus MUCL 29819.” Bioresource technology 274 (2019): 272-280.

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  9. Liu, Jia’nan, et al. “Efficient bioconversion of high-content volatile fatty acids into microbial lipids by Cryptococcus curvatus ATCC 20509.” Bioresource technology 239 (2017): 394-401.

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  10. Huang, Xiang-Feng, et al. “Enhanced biodiesel production from glucose-fed activated sludge microbial cultures by addition of nZVI and FeCl 3.” RSC Advances 6.91 (2016): 88727-88735.

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  11. Liu, Jia, et al. “Bioconversion of volatile fatty acids derived from waste activated sludge into lipids by Cryptococcus curvatus.” Bioresource Technology 211 (2016): 548-555.

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  12. Xu, Jingcheng, et al. “Application of de-lignified cellulose to enhance intracellular and extracellular lipid production from oleaginous yeast using acetic acid.” Bioresource Technology 293 (2019): 122032.

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  13. Bhavsar, Yash, and Yajamana Ramu. “Investigating the effects of mutations of amino acids on the protein expression of CDK2 cancer gene.”

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  14. Killermann, Berta, et al. “Quantitativer und qualitativer Nachweis von Brandkrankheiten (Tilletia spp., Ustilago nuda) bei Weizen und Gerste mittels biotechnologischer Methoden (q-PCR, LAMP-Technologie).” (2021).

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  15. Huang, Xiang-feng, et al. “Enhancement of extracellular lipid production by oleaginous yeast through preculture and sequencing batch culture strategy with acetic acid.” Bioresource Technology 247 (2018): 395-401.

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  16. Leet, Killu. L-ascorbic acid (vitamin C) production by bioengineered Saccharomyces cerevisiae. MS thesis. Eesti Maaülikool, 2022.

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  17. De Biaggi, Juliano Sabedotti. “MODIFICAÇÃO GÊNICA E MODELAGEM METABÓLICA.” (2023).

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SF References for SuperFastPrep-2OEM MonoLyser · distributed by MP Biomedicals 58 references
  1. Schang, Christelle, et al. “A low-cost homogenizing device for in-field and remote DNA and RNA extraction.” HardwareX 20 (2024): e00592.

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  2. Barber, Daniel G., et al. “Statistical design approach enables optimised mechanical lysis for enhanced long-read soil metagenomics.” Scientific Reports 14.1 (2024): 28934.

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  3. Heavens, Darren, et al. “How low can you go? Driving down the DNA input requirements for nanopore sequencing.” BioRxiv (2021): 2021-10.

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  4. Villavicencio-Carrisoza, Oscar, et al. “Isolation of total DNA from Placenta Samples, both Fresh and following Formalin and Paraffin Treatment.” Maternal Placental Interface: Methods and Protocols. New York, NY: Springer US, 2024. 171-178.

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  5. Maggiori, Catherine, et al. “MinION sequencing from sea ice cryoconites leads to de novo genome reconstruction from metagenomes.” Scientific reports 11.1 (2021): 21041.

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  6. Bian, Kaiqin, et al. “Quantitative metagenomics using a portable protocol.” bioRxiv (2025): 2025-10.

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  7. Martínez-Herrera, Sayra Cecilia, et al. “Check Chapter 15 updates for.” Maternal Placental Interface: Methods and Protocols 2781 (2024): 163.

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  8. Edwards, Arwyn, et al. “Before you go: a packing list for portable DNA sequencing of microbiomes and metagenomes.” Microbiology (United Kingdom) 168.7 (2022): 001220.

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  9. Wilkerson-Vidal, Quiana C., et al. “Male LEW. 1WR1 rats develop metabolic dysfunction, steatohepatitis, and liver damage.” Endocrines 5.2 (2024): 166-185.

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  10. Lee, Sang-woo, et al. “Developmental role of hyaluronic acid and its application in salivary gland tissue engineering.” Acta biomaterialia 115 (2020): 275-287.

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  11. Zelentsova, Ekaterina A., Vadim V. Yanshole, and Yuri P. Tsentalovich. “A novel method of sample homogenization with the use of a microtome-cryostat apparatus.” RSC advances 9.65 (2019): 37809-37817.

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  12. Hong, Heesun, et al. “Digital light processing 3D printed silk fibroin hydrogel for cartilage tissue engineering.” Biomaterials 232 (2020): 119679.

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  13. Wen, Jianfeng, Mark W. LeChevallier, and Wendong Tao. “Nitrification kinetics and microbial communities of activated sludge as a full-scale membrane bioreactor plant transitioned to low dissolved oxygen operation.” Journal of cleaner production 252 (2020): 119872.

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  14. Regan, Tim, et al. “Non-invasive detection method for Bonamia ostreae infected Ostrea edulis.” Aquaculture 599 (2025): 742153.

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  15. Ajiteru, Olatunji, et al. “A digital light processing 3D printed magnetic bioreactor system using silk magnetic bioink.” Biofabrication 13.3 (2021): 034102.

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  16. Lee, Ji Seung, et al. “3D-printable photocurable bioink for cartilage regeneration of tonsil-derived mesenchymal stem cells.” Additive Manufacturing 33 (2020): 101136.

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  17. Park, Hae Sang, et al. “Fluidic integrated 3D bioprinting system to sustain cell viability towards larynx fabrication.” Bioengineering & Translational Medicine 8.2 (2023): e10423.

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  18. Wen, Jianfeng, et al. “Nitrous oxide emission and microbial community of full-scale anoxic/aerobic membrane bioreactors at low dissolved oxygen setpoints.” Journal of Water Process Engineering 38 (2020): 101654.

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  19. Wen, Jianfeng, Mark W. LeChevallier, and Wendong Tao. “Microbial community similarity and dissimilarity inside and across full-scale activated sludge processes for simultaneous nitrification and denitrification.” Water science and technology 81.2 (2020): 333-344.

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  20. Touchette, David, et al. “Microbial characterization of Arctic glacial ice cores with a semiautomated life detection system.” Astrobiology 23.7 (2023): 756-768.

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  21. Kim, Soon Hee, et al. “Rapidly photocurable silk fibroin sealant for clinical applications.” NPG Asia Materials 12.1 (2020): 46.

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  22. Andersen, Martin Hjorth, et al. “Fast DNA-analyses for surveillance of microbial communities in full-scale deammonification tanks: Potential for control and troubleshooting.” Water Research 236 (2023): 119919.

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  23. Ivanov, L. A., et al. “Shifts in Pigment Content along an Arid Altitudinal Gradient as a Mechanism for the Regulation of the Photosynthetic Capacity of Plants in the Gobi.” Contemporary Problems of Ecology 18.5 (2025): 698-707.

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  24. Ziganshina, Elvira E., et al. “Bacterial communities associated with atherosclerotic plaques from Russian individuals with atherosclerosis.” PloS one 11.10 (2016): e0164836.

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  25. Hwang, Geumbit, Hyejin Seo, and Joo-Cheol Park. “Copine7 deficiency leads to hepatic fat accumulation via mitochondrial dysfunction.” Heliyon 9.11 (2023).

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  26. Lee, Ji Seung, et al. “Hybrid 3D bioprinting for advanced tissue-engineered trachea: merging fused deposition modeling (FDM) and top–down digital light processing (DLP).” Biofabrication 17.1 (2024): 015026.

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  27. Sinigalliano, Christopher, et al. “Microbial source tracking of fecal indicating bacteria in coral reef waters, recreational waters, and groundwater of Saipan by real-time quantitative PCR.” Frontiers in Microbiology 11 (2021): 596650.

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  28. Kim, Junchul, Sang-Woo Lee, and Kyungpyo Park. “CXCR4 regulates temporal differentiation via PRC1 complex in organogenesis of epithelial glands.” International Journal of Molecular Sciences 22.2 (2021): 619.

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  29. Yarullina, Dina R., et al. “Characterization of gut contractility and microbiota in patients with severe chronic constipation.” PLoS One 15.7 (2020): e0235985.

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  30. Wilkerson-Vidal, Quiana C., et al. “Insulin-Resistant Male LEW. 1WR1 Rats Do Not Develop β-Cell Mass Expansion in Response to a Moderate Sucrose Diet.” Journal of Molecular Pathology 5.3 (2024): 276-291.

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  31. Zhang, Tao, et al. “Diversity and distribution of lichen-associated fungi in the Ny-Ålesund Region (Svalbard, High Arctic) as revealed by 454 pyrosequencing.” Scientific Reports 5.1 (2015): 14850.

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  32. Pallen, Mark J., et al. “Faecal metagenomes of great tits and blue tits provide insights into host, diet, pathogens and microbial biodiversity.” Access Microbiology 7.4 (2025): 000910-v3.

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  33. Gopaulchan, David, et al. “A defined microbial community reproduces attributes of fine flavour chocolate fermentation.” Nature Microbiology (2025): 1-23.

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  34. Khadair, Sana Salman. “NMR Analysis of Metabolomic Changes in Levels of Neurotransmitters in Rodents.” (2019).

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  35. Ziganshina, Elvira E., et al. “Comparison of anaerobic digestion strategies of nitrogen-rich substrates: performance of anaerobic reactors and microbial community diversity.” Waste Management 59 (2017): 160-171.

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  36. Lee, Sang-woo, et al. “Efficient surface immobilization of chemically modified hyaluronans for enhanced bioactivity and survival of in vitro-cultured embryonic salivary gland mesenchymal cells.” Polymers 13.8 (2021): 1216.

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  37. de Menezes, Alexandre Barretto, et al. “Relationships between radiation, wildfire and the soil microbial communities in the Chornobyl Exclusion Zone.” Science of the Total Environment 950 (2024): 175381.

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  38. 이상우. Biomimetic Approaches for Tissue Engineering Based on the Roles of Hyaluronic Acid in Salivary Gland Development. Diss. 서울대학교 대학원, 2021.

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  39. Zhang, Tao, et al. “Diversity and distribution of fungal communities in the marine sediments of Kongsfjorden, Svalbard (High Arctic).” Scientific reports 5.1 (2015): 14524.

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  40. Смирнов, А. В., et al. “Получение трансгенных линий мышей для изучения возможности конверсии лактозы в галактоолигосахариды в молочной железе.” ПИСЬМА В ВАВИЛОВСКИЙ ЖУРНАЛ ГЕНЕТИКИ И СЕЛЕКЦИИ Учредители: Федеральный исследовательский центр институт цитологии и генетики СО РАН 7.4 (2021): 176-193.

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  41. Child, Harry T., et al. “Laboratory and In-Field Metagenomics for Environmental Monitoring.” Genome Assembly. New York, NY: Springer US, 2025. 71-88.

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  42. Sindhu, Lara, et al. “Meta-omics revealed that nitrogen fertilization alters the endophytic fungi divergence in maize field ecosystem.” Ecological Indicators 160 (2024): 111852.

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  43. Sondhiya, Shubhank. The robotic molecular biologist-automated processing of astrobiological samples for planetary missions. Diss. Carleton University, 2020.

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  44. Sambasivam, Prabhakaran, et al. “A rapid electrochemical biosensor diagnostic for Botrytis ssp. causing Botrytis gray mold of temperate legumes.” ACS Agricultural Science & Technology 4.11 (2024): 1184-1193.

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  45. Zhang, Tao, and Yi-Feng Yao. “Endophytic fungal communities associated with vascular plants in the high arctic zone are highly diverse and host-plant specific.” PLoS One 10.6 (2015): e0130051.

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  46. Zhang, Tao, Neng-Fei Wang, and Li-Yan Yu. “Geographic distance and habitat type influence fungal communities in the Arctic and Antarctic sites.” Microbial ecology 82.1 (2021): 224-232.

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  47. Zhang, Tao, et al. “A comprehensive assessment of fungal communities in various habitats from an ice-free area of maritime Antarctica: diversity, distribution, and ecological trait.” Environmental Microbiome 17.1 (2022): 54.

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  48. Arslanova, Alisa, et al. “Protective effects of probiotics on cognitive and motor functions, anxiety level, visceral sensitivity, oxidative stress and microbiota in mice with antibiotic-induced dysbiosis.” Life 11.8 (2021): 764.

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  49. Wimalarathne, Madushika M. Hyperinsulinemic LEW. 1WR1 rats develop insulin resistance and nonalcoholic steatohepatitis. The University of Alabama in Huntsville, 2023.

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  50. 한상인. Synthesis of Bimetallic Nanomaterials for Stretchable Biomedical Device and Radioprotective Agent. Diss. 서울대학교 대학원, 2019.

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  51. Zhang, Tao, Nengfei Wang, and Liyan Yu. “Host-specificity of moss-associated fungal communities in the Ny-Ålesund region (Svalbard, High Arctic) as revealed by amplicon pyrosequencing.” Fungal Ecology 53 (2021): 101092.

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  52. Bade, Pauline, et al. “Integrative analysis of human macrophage inflammatory response related to Mycobacterium tuberculosis virulence.” Frontiers in Immunology 12 (2021): 668060.

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  53. Lamprecht, Dirk A., et al. “Targeting de novo purine biosynthesis for tuberculosis treatment.” Nature (2025): 1-7.

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  54. Knapp, Kiley A., et al. “Evaluation of Sas1B as a target for antibody-drug conjugate therapy in the treatment of pancreatic cancer.” Oncotarget 9.10 (2018): 8972.

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  55. Bautista, Juan Sebastián Osorio. “Desarrollo de un algoritmo predictor de consorcios bacterianos, partiendo de características metabólicas con potencial uso para la biorremediación de aguas residuales contaminadas con cromo hexavalente.”

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  56. Калинин, Егор Валерьевич. “БИОМЕДИЦИНСКИЙ ПОТЕНЦИАЛ ФАКТОРА ПАТОГЕННОСТИ LISTERIA MONOCYTOGENES INLB.”

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  57. Badé, Pauline. Étude du rôle de la réponse inflammatoire et des microARN du macrophage au cours de l’infection avec Mycobacterium tuberculosis. Diss. Université Montpellier, 2021.

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  58. Harris, Shelley Elizabeth. Effects of hypothyroidism on pancreatic islet development and tissue insulin signalling pathways in the ovine fetus. Diss. Oxford Brookes University, 2016.

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About this library: The supplied list includes peer-reviewed articles alongside protocols, chapters, preprints, theses and other scholarly sources. Inclusion indicates reported use or citation of the platform; it does not imply RotaPrep authorship or endorsement.

External source and patent links open in a new tab. Bibliographic details reflect the supplied reference list.

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